template reaction

Using the DNA extracted from bone marrow smears which have been stored routinely as a template, polymerase chain reaction ( PCR) was utilized to detect clonal immunoglobulin heavy chain ( IgH) gene rearrangements in multiple myeloma ( MM) and reactive plasmacytosis ( RP).

从常规保存的骨髓涂片抽提DNA为模板，应用聚合酶链反应（PCR）技术检测多发性骨髓瘤（MM）与反应性浆细胞增多症（RP）IgH基因重排方式。

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Through coordinating organic ligands, metal particles, template, and controlling reaction conditions, organic skeleton materials of porous metal will be likely produced.

通过调节有机配体、金属离子、模板剂和控制反应条件可能定向组装出预期的多孔金属有机骨架材料。

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Also attended with a hard template to the reaction mixture, the hierarchical materials containing micro-, meso-and macropores were obtained after completing the reaction and removing the hard template.

向反应中加入硬模板，反应完成后洗脱模板，得到了同时含有微孔、介孔和大孔的多级孔材料。

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Then the template substrate reaction with different boric acid, boric acid and ester reaction of pyridine, the corresponding yield obtained to study the law.

以此优化的条件为前提，再将该模板反应的底物与不同的硼酸，吡啶硼酸和酯反应，得出相应的产率，研究其规律。

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S A method of rapid detecting Leptospira DNA was constructed after the template DNA from 4 serotypes of Leptospira interrogans were successfully amplified by polymerase chain reaction ( PCR).

以四株不同型别的致病性钩端螺旋体DNA为模板进行聚合酶链反应（PCR），建立了钩体快速检测方法。

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CD was cultured successfully, and the genome was extracted as template of PCR reaction. 2.

获得如下结果；1.成功的培养了艰难梭菌，并提取了其基因组作为PCR反应模板。

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And the catalysts for the reaction were optimized through comparison of experimental template reaction.

并通过对模板反应的对比实验，对反应中加入的催化剂进行了优化。

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The optimum annealing temperatures of 51 ° C-55 ° C were definite after selecting and optimization of the primer combinations for SRAP with the DNA pools from male and female plants of Ginkgo as a template in the stable reaction system obtained. 5.

以银杏的雌雄株DNA池为模板，用获得的稳定的反应体系对sRAP引物组合进行筛选优化，得到不同引物组合的最适退火温度，退火温度的范围在5l℃-55℃之间。

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Attended with solid nanoparticles ( size greater than50nm) as hard template in the reaction mixture, the hierarchical porous materials containing micro-, meso-and macro-pores were obtained.

与此同时通过在合成体系中加入固体物质纳米粒子（粒度大于50nm）作为硬模板，在除去模板后，获得了同时具有微孔、介孔和大孔的多级孔材料。

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In this part, a novel fluorescent method of protein detection has was developed using hairpin aptamer based on polymerase reaction. The hairpin aptamer was designed to be employed as the protein ligand and template of the polymerase reaction.

本部分设计了一种新型的发夹型核酸适体探针，利用其与靶蛋白反应后构象发生变化的特点，建立了一种基于聚合酶反应的荧光信号放大的蛋白质检测新方法。

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This model describes the function relation between product quantity or fluorescence intensity and initial template number and other reaction conditions, and can reflect the accumulating rule of PCR product molecule accurately.

PCR动力学数学模型是根据PCR技术的原理提出的，能够准确描述PCR反应产物分子数量积累规律的动力学方程，给出了PCR产物数量或者荧光强度与初始模板数量及其他反应条件间的函数关系。

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